Details major developments in the practical applications of High Performance Liquid Chromatography. An essential resource for chromatographers who wish to improve their techniques in HPLC research, this collection of articles by specialists in the field presents both a broad overview of recent developments as well as more detailed knowledge in areas of chromatography involving biotechnology. Different analytical techniques required for the detection of variants are described, as is the transformation of an analytical into a preparative separation. Mass spectroscopy and related technologies used for structural characterization are discussed. Together, the contributions provide a complete picture of this exciting and rapidly moving field.