Forty chapters deal with various aspects of tissue culture, in vitro manipulation, and other biotechnological approaches to the improvement of maize. They are arranged in eight sections; - In Vitro Technology, Callus Cultures and Regeneration of Plants, Somatic Embryogenesis. - Wide Hybridization, Embryo, Ovule, and Inflorescence Culture, in Vitro Fertilization. - Production of Haploids and Double Haploids, Anther and Pollen Culture. - Protoplast Culture, Genetic Transformation. - Somaclonal Variation and Mutations. - Molecular Biology and Physiological Studies. - Proteins and Nutritional Improvement. Pollen Storage, Cryopreservation of Germplasm.